Figure 4

Estren induces increased submandibular gland weight and androgen phenotype independently of estrogen receptors. Ovariectomized 3-month-old female C57/B16 mice were treated for 18–21 days with daily s.c. injections of the estrogen receptor antagonist ICI 182,780 (200 μg/day) or vehicle Miglyol 812. Simultaneously, both the vehicle and the ICI groups were given daily s.c injections of either E2 (0.7 μg/day), estren (75 μg/day) or control Miglyol 812 oil. Treatment with E2 did not affect the SMG weight (A) or score for androgen phenotype (B) in either vehicle or ICI mice. Estren-treatment resulted in increased SMG weight (A) and SMG score for androgen phenotype (B) in both vehicle and ICI mice. One-way ANOVA followed by Fisher's test was used to compare data from mice in different treatment groups (A). Results are presented as mean ± standard deviation. **P < 0.01, *** P < 0.001.