Figure 5

DHT and estren reduce the frequency of B220⁺ cells bone marrow independently of estrogen receptors. Ovariectomized 11-month-old female WT and DERKO mice were given daily s.c injections of E2 (0.7 μg/day), DHT (120 μg/day) or estren (75 μg/day) during four weeks. Control mice received olive oil. As expected, the E2 mediated reduction of B220⁺ cells in bone marrow of WT mice was lacking in DERKO mice. Treatment with DHT or estren resulted in a lower frequency of B220⁺ cells in bone marrow of both WT and DERKO mice. One-way ANOVA followed by Fisher's test was used to compare data from mice in different treatment groups. Results are presented as mean ± standard deviation. * P < 0.05, * *P < 0.01, *** P < 0.001.