Figure 1
From: Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT
Representative data from the three assays on the same CMV seropositive donor PBMC. (A) Tetramer staining after gating as described in the Methods. Background seen in staining of fresh cells with negative tetramer was caused by streptavidin-PE. Frozen cells were stained with a different lot of negative tetramer. (B) CFC staining after gating as described in the Methods. (C) ELISPOT data as analyzed on the Automated Reader System as described in the Methods. Error bars represent the S.D. of 6 replicates.