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Table 1 Cutoffs, sensitivity, and specificity from ROC curves

From: Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT

Assay

Antigen

Sample Type

Cutoff Point1

Sensitivity

Specificity

Area Under ROC Curve2

Tetramer (% +)

CMV pp65 A2 peptide

Fresh

0.05

92%

88%

0.906+/-0.076

  

Cryo.

0.02

83%

100%

0.875+/-0.086

 

CMV pp65 peptide mix

Fresh

0.13

90%

71%

0.819+/-0.075

CFC (% +)

 

Cryo.

0.05

90%

76%

0.920+/-0.046

 

CMV pp65 A2 peptide

Fresh

0.08

75%

100%

0.844+/-0.084

  

Cryo.

0.08

83%

88%

0.891+/-0.074

ELISPOT (# SFC)

CMV pp65 peptide mix

Fresh

4

95%

94%

0.982+/-0.018

  

Cryo.

16

90%

100%

0.985+/-0.015

  1. 1Cut-off is that which achieves maximum specificity for sensitivity ≥90%, or that which obtains maximum sensitivity if maximum sensitivity <90%.)
  2. 2Values are given +/- standard error.