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Figure 10 | BMC Immunology

Figure 10

From: Quantitative PCR for detection of the OT-1 transgene

Figure 10

Detection of OT-1 Cells from Archival Material. Detection of OT-1 cells from paraffin-embedded archived material is possible using the OT-1 qPCR assay. Paraffin embedded liver sections (5 uM) of C57BL/6 mice used as hosts in OT-1 CD8+ T cell adoptive transfer experiments were used to generate template DNA. The animals were sacrificed on day five after the first i.p injection of the stimulating OVA peptide or PBS control.

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BMC Immunology

ISSN: 1471-2172

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