Figure 2

Chemotactic responses of cells to metallothionein in the modified Boyden chamber assay. A. Mouse splenocytes or Thioglycollate-elicited cells respond to guinea pig serum (as a source of activated complement) and to metallothionein. Cells that had migrated to the lower side of the filters were counted in at least six fields of view per well. Data are means of six fields in each of six replicates. The data shown is representative of three experiments and is expressed as the average ± standard deviation. B. Dose response curve of chemotaxis to metallothionein. Different concentrations of metallothionein were loaded into the lower wells of the Boyden chambers and Jurkat T cells were added to the upper wells.