Figure 5

Inhibitors of MT chemotaxis. A: Monoclonal anti-metallothionein antibody blocks the chemotactic response of Jurkat T cells to 20 μM metallothionein gradient. Affinity-purified metallothionein (20 μM) was incubated with antibody (clone UC1MT or E9) and then added to the chemoattractant well. Controls were performed with metallothionein alone (MT), medium alone (-), or metallothionein preincubated with isotype-matched control antibody (not shown). B: Cholera toxin (CTX) inhibits chemotactic response to metallothionein. Jurkat T cells were preincubated with or without 0.133 μg/10⁶ cells/ml CTX at 37°C for 1 h and then added to the cell well. Chemotactic responses of toxin-treated cells to MT (CTX-MT) were compared to untreated cells (MT) and untreated cells without an MT gradient (-). C: Pertussis toxin (PTX) blocks the chemotactic response to metallothionein. Jurkat T cells were preincubated with or without 200 ng/10⁶ cells/ml PTX at 37°C for 16 h. Chemotactic responses of toxin-treated cells to MT (PTX-MT) was compared to untreated cells (MT) and untreated cells without an MT gradient (-). Each figure is a representative more than four independent experiments performed in triplicate.