Table 1 DIAS analysis of chemokinetic and chemotactic movement. Jurkat T cells were cultured under agarose in the presence of uniform concentrations or in a gradient of the stimuli. Cells were imaged over time and their motile behavior quantified. Persistence is an indicator of the rate of directional change. Chemotactic index is a measure of the proportion of movement in a designated direction (1 = toward a source, 0 = random movement, -1 = away from the source).
Stimulus (n = number of analyzed cells) | Average translational speed (μm/min) | Persistence (μm/minute-degree) | Chemotactic index |
|---|---|---|---|
Media control (7) | 0.66 ± 0.29 | 0.18 ± 0.11 | 0.19 |
Uniform concentration of metallothionein (10) | 2.110 ± 0.73 | 0.5 ± 0.22 | 0.07 |
Uniform concentration of SDF-1α (10) | 1.69 ± 0.82 | 0.53 ± 0.34 | 0.09 |
Metallothionein gradient (7) | 6.6 ± 3.2 | 2.042 ± 1.09 | 0.73 |
SDF-1α gradient (7) | 3.31 ± 1.48 | 1.17 ± 0.49 | 0.72 |