Follicular dendritic-like cells derived from human monocytes

BMC Immunology

Table 1 Phenotype and function of primary and secondary FDC-like cells as compared with macrophages generated in vitro and with freshly prepared monocytes

	Monocytes [day 0]	Macrophages [day 15]	Primary FDC-like cells [day 15]	Secondary FDC-like cells [day 18]
HLA-DR	+	++	+	++
CD22	-	-	±	±
CD35 (CR1)	+	+	+++	+++
CD45	+++	+++	±	N.D.
CD54 (ICAM-1)	+	-	-	++
CD68	++	+++	+±	+±
CD106 (VCAM-1)	-	-	-	+
AP	-	-	+++	+++
Clustering.	-	-	++	+++
B cell Rosetting	-	-	+++	+++
Emperipolesis	-	-	++	++
Antigen Retention	-	-	+++	+++

Markers and functions are expressed as absent (-), faintly or few positive cells (+), positive with different intensities (+, ++, +++), variable with respect to donor variations (±). The term, clustering, refers to the ability to establish homotypic and heterotypic aggregates. N.D. not determined.

ISSN: 1471-2172