Skip to main content

Table 3 Intergenic difference of RT efficiencies is not due to interassay variation. Serial 1:10 dilutions of a single cellular RNA sample, of a pooled RNA sample and of a pooled DNA sample were used to generate standard curves for different targets (TNF-α, β2-MG, MIF and UBC). Slopes were indicative of amplification efficiencies, which were normalised to 100% for the pooled DNA sample for easier comparison to amplification efficiencies of the two other samples.

From: CyProQuant-PCR: a real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use

  Single cellular RNA sample Pooled RNA sample Pooled DNA sample
TNF-α 95 95 100
β2-MG 90 90 100
MIF 88 88 100
UBC 81 80 100