Table 5 Expression stability of endogenous standard genes under non-normalised conditions After 3 hours of stimulation with LPS, PBMC from healthy donors were split in 2 identical aliquots and total cellular RNA was extracted at 2 days interval. The same volume of RNA was reverse transcribed and β2-MG, UBC and YWHAZ transcripts were amplified by real time PCR using CyProQuant-PCR technique. Results show the mean values obtained for the 2 extractions in arbitrary units.
Stimulation conditions | Molecules detected (arbitrary units) | |
|---|---|---|
T0 | LPS 3 h | |
β2-MG | 1.24 107 | 1.48 107 |
(1.00 107 – 1.49 107) | (1.17 107 – 1.79 107) | |
UBC | 2.04 105 | 2.39 105 |
(0.62 105 – 3.46 105) | (1.51 105 – 3.28 105) | |
YWHAZ | 1.89 105 | 2.80 105 |
(0.78 105 – 3.01 105) | (2.25 105 – 3.34 105) | |