Figure 4

The CCR3/EGFP transgenic mice. In (A), a schematic representation of the transgenic construct is shown. The genomic fragment encoding 1.6 kb of the CCR3 promoter including 60 bp of exon 1 (E1), was cloned into the Hind III and Bam HI sites upstream from the EGFP gene in the pEGFP1 vector. The positions of restriction sites are indicated. In (B), Northern blot analysis of multiple tissues from transgenic mice is shown. RNA was isolated from the spleen, lung, thymus, kidney and jejunum of CCR3/EGFP transgenic mice (lines 3.1 and 3.2). 10 μg total RNA was electrophoresed on an agarose gel, transferred to nylon membranes and probed with the SV40 polyA probe in order to detect expression of the transgene. Position of 28S and 18S is depicted with arrows. Ethidium bromide staining of the gel is also shown. In (C), immunohistochemistry using an anti-GFP antibody is shown. Organs were collected, frozen sections obtained and anti-GFP immunohistochemistry performed. Positive staining is represented as black precipitate.