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Figure 7 | BMC Immunology

Figure 7

From: BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1

Figure 7

BMP-6 induced upregulation of Id1 at the mRNA and protein level. CD19+ B cells were cultured in X-vivo 15 over night before treatment with BMP-6 for the indicated time points. Total RNA was extracted and Id1, Id2 or Id3-expression was analysed by real-time RT-PCR; values are normalised to the expression level of PGK1 mRNA and expressed as relative quantification (2-average ΔΔCT – relative to Id1, Id2 or Id3 expression in the cell-line Ramos). One representative of three independent experiments is shown.

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