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Figure 4 | BMC Immunology

Figure 4

From: VACUTAINER® CPT™ and Ficoll density gradient separation perform equivalently in maintaining the quality and function of PBMC from HIV seropositive blood samples

Figure 4

Processing method does not adversely impact functional response. IFNγ expression by CD8+ and CD8- T Cells, in response to 6 hour activation with either CMVpp65 peptide mix (A and C) or HIVp55 peptide mix (B and D), was used as a read-out of functional ability of the PBMC. Data displayed have been corrected for the unstimulated expression of cytokine. For FACS analysis, a lymphocyte (forward vs. side scatter) gate, and a CD3+CD8+ gate (A and B) or a CD3+CD8- gate (C and D) were applied. Significant difference (p) was determined by the Wilcoxon signed rank test. Each filled circle represents an individual donor and each donor is represented by the same color for each of the conditions tested. The "+" represents the median of all the donors in the category.

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BMC Immunology

ISSN: 1471-2172

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