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Figure 5 | BMC Immunology

Figure 5

From: VACUTAINER® CPT™ and Ficoll density gradient separation perform equivalently in maintaining the quality and function of PBMC from HIV seropositive blood samples

Figure 5

Antigen-specific stimulation of T cells results in cytokine expression. Intracellular staining with IFNγ FITC/CD69 PE/CD8 PerCPCy5.5/CD3 APC by Ficoll or CPT-processed PBMC from one representative HIV+ donor. The numbers displayed on the dot-plots represent the percentage of CD3+ and CD8+ lymphocytes that are expressing IFNγ and CD69 in response to antigen-specific stimulation or unstimulated control.

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BMC Immunology

ISSN: 1471-2172

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