Figure 2

Induction of PNAd in arthritic synovium at day 3. Sections of knee or ankle joint synovial tissue (obtained at days 3, 9, and 15) and control tissue (from same times and joints) were stained with a CD31 antibody (Cy-2, green), MECA-79 (Cy3, red) and with hematoxylin for bright field visualization. The depicted two-color fluorescence micrographs (400-fold magnification) were taken on sections from day 3 and are representative for the other sections from this time. Sections from days 9 and 15 of the model stained similarly; however, the staining intensity with MECA-79 and the fraction of vessels staining with MECA-79 appeared to be somewhat less than in sections from day 3 (quantitative image and statistical analysis was not performed).