Table 2 Primers and probes used for TaqMan Q-PCR

mouse GlcNAc6ST-1 (GeneBank accession no.NM_018763):

forward primer

5' CAAGCGGCAGTTGGTGTATGTGTT 3',

reverse primer

5' TTCAGGGCAAACTGCTCCATTTCG 3',

Taqman probe:

5'ACAGTATGGCCAAGACGCTGCAAACA 3'.

mouse GlcNAc6ST-2 (GeneBank accession no.NM_011998):

forward primer

5' ATCTTCTGCGTTCCGTCTTCCTGT 3',

reverse primer

5' TTCCTGGCGTTAGTATGGAAGGCA 3',

Taqman probe:

5'TGTGCTAGGGCAGCATTTGGAAACGA 3'.

mouse GAPDH (GeneBank accession no.NM_001001303):

forward primer

5' GAATGGGAAGCTTGTCATCAACGG 3',

reverse primer

5' TAGACTCCACGACATACTCAGCAC 3',

Taqman probe:

5'AAGCCCATCACCATCTTCCAGGAGCGAGA 3'.

mouse IL-1β (GeneBank accession no.NM_008361):

forward primer

5' GTACAAGGAGAACCAAGCAACGAC 3',

reverse primer

5' GTGCCGTCTTTCATTACACAGGAC 3',

Taqman probe:

5'ACCTGTGGCCTTGGGCCTCAAAGGAAAGAA 3'.

mouse IL-10 (GeneBank accession no.NM_010548):

forward primer

5' ACTGCTATGCTGCCTGCTCTTACT 3',

reverse primer

5' TGGCCTTGTAGACACCTTGGTCTT 3',

Taqman probe:

5'AAGCATGGCCCAGAAATCAAGGAGCA 3'.