Figure 3

Wnt3A induces β-catenin stabilization in BM B progenitor cells. Western blot analysis of β-catenin levels in BM CD10⁺ B lineage progenitor cells stimulated with Wnt3A (100 ng/ml) or vehicle (PBS with 0.1% detoxified BSA) for 3 hours. The blots were incubated with an Ab against β-catenin, followed by an Ab against β-actin to ascertain equal loading in the wells. The same results were found in cells from 4 out of 5 different donors, indicating some degree of donor variation in the response to Wnt3A.