Wnt expression and canonical Wnt signaling in human bone marrow B lymphopoiesis

BMC Immunology

Table 2 Number of CD19 cells after two weeks of culturing BM CD10⁺ cells on stromal cells

Exp. No. (with MS5)	Control-CM	Wnt3A-CM	Inhibition Index*
1	2182 ± 427	184 ± 91	0.08
2	9440 ± 1953	2652 ± 721	0.28
3	7292 ± 1928	2524 ± 475	0.35
Exp. No. (with BMS)	Medium	rmWnt3A	Inhibition Index*
1	1746 ± 300	920 ± 64	0.53

BM CD10⁺ cells were cultured on a layer of the murine stromal cell line MS-5 in the presence of Wnt3A-conditioned medium (Wnt3A-CM) or control-conditioned medium (control-CM), or on a layer of human bone marrow stromal cells (BMS) in the presence of rmWnt3A or control medium. The numbers in the table represent the mean of duplicate wells ± SD. *Number of CD19⁺ cells in wells containing Wnt3A divided by number of cells in wells containing control-medium.

ISSN: 1471-2172