Table 3 Primer sequences used for mRNA expression analyses of Wnt ligands, Fzd receptors and Wnt antagonists
From: Wnt expression and canonical Wnt signaling in human bone marrow B lymphopoiesis
Primer | Sequence | Amplicon (bp) |
|---|---|---|
Wnt1 | F-5' TAG CCT CCT CCA CGA ACC TG-3' | 239 |
| Â | F-5' CAG CCT CGG TTG ACG ATC TTG-3' | Â |
Wnt2 | F-5' TGG TGG TAC ATG AGA GCT ACA GGT G-3' | 297 |
| Â | R-5' CCC TGG TGA TGG CAA ATA CAA C-3' | Â |
Wnt2B | F-5' TCA TGC TCA GAA GTA GCC GAG A -3' | 328 |
| Â | R-5' TGG CAC TTA CAC TCC AGC TTC A -3' | Â |
Wnt3 | F-5' CTG GCT ACC CAA TTT GGT GGT-3' | 225 |
| Â | R-5' CAT CTA TGG TGG TGC AGT TCC A-3' | Â |
Wnt3A | F-5' AAG CAG GCT CTG GGC AGC TA-3' | 234 |
| Â | R-5' GAC GGT GGT GCA GTT CCA-3' | Â |
Wnt4 | F-5' GAG GAG ACG TGC GAG AAA CTC AA-3' | 346 |
| Â | R-5' ATC CTG ACC ACT GGA AGC CCT GT-3' | Â |
Wnt5A | F-5' ATC CTG ACC ACT GGA AGC CCT GT-3' | 358 |
| Â | R-5' GGC TCA TGG CGT TCA CCA C-3' | Â |
Wnt5B | F-5' CAG CTT CTG ACA GAC GCC AAC T-3' | 279 |
| Â | R-5' GCC TAT CTG CAT GAC TCT CCC A-3' | Â |
Wnt6 | F-5' GCT CCA GCC ACA GCA AGG-3' | 378 |
| Â | R-5' CAG CCT GCC CGC CTC GTT-3' | Â |
Wnt7A | F-5' CCT GGG CCA CCT CTT TCT CAG-3' | 573 |
| Â | R-5' TCC AGC TTC ATG TTC TCC TCC AG-3' | Â |
Wnt7B | F-5' TTT CTC TGC TTT GGC GTC CT-3' | 391 |
| Â | R-5' TGG TTG TAG TAG CCC TGC TTC TC-3' | Â |
Wnt8A | F-5' TCC CAA GGC CTA TCT GAC CTA C-3' | 400 |
| Â | R-5' CCG GCC CTG TTG TTG TGA-3' | Â |
Wnt8B | F-5' GCC CAG AGT GGT ATT GAA GAA TG-3' | 266 |
| Â | R-5' TTG TCA CTG CAG CCT CCC-3' | Â |
Wnt9A | F-5' AAG TAC AGC AGC AAG TTC GTC AAG G-3' | 538 |
| Â | R-5' GCA CTC CAC ATA GCA GCA CCA AC-3' | Â |
Wnt9B | F-5' AGT TTC AGT TCC GGC ATG AGC-3' | 340 |
| Â | R-5' TTC ACA GCC TTG ATG CCC A-3' | Â |
Wnt10A | F-5' ACA CAG TGT GCC TAA CAT TGC C-3' | 296 |
| Â | R-5' AGG CCT TCA GTT TGC CCA G -3' | Â |
Wnt10B | F-5' CCT CGC GGG TCT CCT GTT C-3' | 563 |
| Â | R-5' GGT TAC AGC CAC CCC ATT CC-3' | Â |
Wnt11 | F-5' ACA ACC TCA GCT ACG GGC TCC T-3' | 394 |
| Â | R-5' CCC ACC TTC TCA TTC TTC ATG C-3' | Â |
Wnt16 | F-5' CTG TGC AAG AGG AAA CCG TAC CTG-3' | 520 |
| Â | R-5' CAG CAC AGG AGC CGG AAA CT-3' | Â |
Fzd1 | F-5' CTC TAC TTC TTC AGC ATG GCC A-3' | 230 |
| Â | R-5' TCC ACG TTG TTA AGC CCC A-3' | Â |
Fzd2 | F-5' CCA TCC TAT CTC AGC TAC AAG TTT CT-3' | 306 |
| Â | R-5' GCA GCC CTC CTT CTT GGT-3' | Â |
Fzd3 | F-5' TCC CCT CTG CCT GTA TGT GGT AGT-3' | 622 |
| Â | R-5' GCT GCT CAC TTT GCT GTG GA-3' | Â |
Fzd4 | F-5' CTC GGC TAC AAC GTG ACC AAG AT-3' | 605 |
| Â | R-5' AAT ATG ATG GGG CGC TCA GGG TA-3' | Â |
Fzd5 | F-5' GTG CCC ATT CTG AAG GAG TCA C-3' | 197 |
| Â | R-5' TCC ATG TCG ATG AGG AAG GTG-3' | Â |
Fzd6 | F-5' ACT CTT GCC ACT GTG CCT TTG-3' | 300 |
| Â | R-5' GTC GAG CTT TTG CTT TTG CCT-3' | Â |
Fzd7 | F-5' CAA GAC CGA GAA GCT GGA GAA G-3' | 248 |
| Â | R-5' TGC CGA CGA TCA TGG TCA T-3' | Â |
Fzd8 | F-5' GGA CTA CAA CCG CAC CGA CCT-3' | 407 |
| Â | R-5' ACC ACA GGC CGA TCC AGA AGA C-3' | Â |
Fzd9 | F-5' TCA AGG TCA GGC AAG TGA GCA-3' | 210 |
| Â | R-5' AGC TTC CAG AGG AAC GCA ACA-3' | Â |
Fzd10 | F-5' CAG GTG TGC AGC CGT AGG TTA A-3' | 212 |
| Â | R-5' AAG CAC CAC ATC TTA GCT CCG G-3' | Â |
WIF1 | F-5' ACG GAC CTC ACT GTG AGA AAG C-3' | 200 |
| Â | R-5' GCT GAT TTC ACA CTG CTC TCC C-3' | Â |
sFRP1 | F-5' GGT CAT GCA GTT CTT CGG CT-3' | 206 |
| Â | R-5' TCC TCA GTG CAA ACT CGC TG-3' | Â |
sFRP2 | F-5' ACC GAG GAA GCT CCA AAG GTA T -3' | 259 |
| Â | R-5' TCA TCT CCT CAC AGG TGC ACT G -3' | Â |
sFRP3 | F-5' CTC ATC AAG TAC CGC CAC TCG TG-3' | 210 |
| Â | R-5' CCG GAA ATA GGT CTT CTG TGT AGC TC-3' | Â |
sFRP4 | F-5' GCA CAT GCC CTG GAA CAT CAC-3' | 243 |
| Â | R-5' ATC TTC ATG AGG GGC TCG CAG T-3' | Â |
Dkk1 | F-5' ACC ATT GAC AAC TAC CAG CCG T -3' | 235 |
| Â | R-5' TGG TTT CCT CAA TTT CTC CTC G -3' | Â |
Dkk4 | F-5' CGT TCT GTG CTA CAT GTC GTG G-3' | 241 |
| Â | R-5' TCT TGT CCC TTC CTG CCT TGT-3' | Â |