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Figure 5 | BMC Immunology

Figure 5

From: Structure function analysis of SH2D2A isoforms expressed in T cells reveals a crucial role for the proline rich region encoded by SH2D2A exon 7

Figure 5

Tyrosines within aa239–334 determines the inhibitory effect of TSAd on Lck activity. The phosphorylation level of LAT and Y319 of Zap-70 in Jurkat T cells expressing the SH2D2A-5 variant and TSAd-4YF is normal upon TCR stimulation: Jurkat T cells were transiently transfected with either pEF-HA or one of the pEF-HA-SH2D2A-1-5 or pEF-HA-TSAd-4YF cDNAs. The cells were stimulated (+) with anti-CD3 (OKT3) mAbs for 2.5 min and lysed. The lysates were immunoblotted with Abs as indicated.

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BMC Immunology

ISSN: 1471-2172

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