Table 1 Primers and probes for quantitative PCR
Oligo | Sequence | Specificity | GeneBank: | Position |
|---|---|---|---|---|
19-FW | 5'-CAT AGT TCC TCT CTG AGA AAC | SH2D2A-3 | AY763098 | 7–27 |
WT-42-FW | 5'-TCA TGG AGT TCC CCC TGG C | SH2D2A-2 | BI820427 | 19–38 |
WT-42-19-RV | 5'-AGC TCC TGC GGG TCA TGT | SH2D2A | AJ000553 | 169–186 |
45-FW | 5'-CTG CCT GGT TCC ATG GCT T | SH2D2A | AJ000553 | 365–383 |
45-RV | 5'-TCC AGC AGC CTC TCT GCC | SH2D2A | AJ000553 | 398–415 |
Ex7-FW | 5'-CCA GTA CAG CCC AAT CAT CAA | SH2D2A | AJ000553 | 728–748 |
Ex7-RV | 5'-TTG CCC AAT CAC AGA GTT CTC A | SH2D2A | AJ000553 | 1115–1136 |
ZAP-70-FW | 5'-ACA CCC TCA ACT CAG ATG GAT ACA | Zap 70 | BC053878 | 1036–1060 |
ZAP-70-RV | 5'-TCG GCC GCG GTT TGT | Zap 70 | BC053878 | 1090–1105 |
Probe-45 | 5'-FAM-TTC GGC CCC CTC TCT CCG TCA CC-DQ | SH2D2A-4 | AY763100 | 397–419 |
Probe-WT-19 | 5'-FAM-GTC ACG AAG CCC CCA TCC CA-DQ | SH2D2A-1,3–5 | AJ000553 | 124–144 |
Probe-42 | 5'-FAM-AGA TAT GTC CCC AAG CAC CTT CCA GAT C-DQ | SH2D2A-2 | BI820427 | 41–77 |
Probe-ex7 | 5'-FAM-AGA AGG AGA ATA CAG GTG GCT CCC AGC-DQ | SH2D2A-5 | AA063001 | 482–509 |
Probe Zap-70 | 5'-HEX-CCC TGA GCC AGC ACG CAT AAC GT-DQ | Zap-70 | BC053878 | 1062–1184 |