Figure 1
From: Enumeration of leukocyte infiltration in solid tumors by confocal laser scanning microscopy
(top row) Indirect immunofluorescence detection of CD3 (T-cells), CD8 (cytotoxic/suppressor T-cells), CD4 (Helper/inducer T-cells) and CD14 (monocyte/macrophage) markers in 20 μm frozen tissue sections from an ovarian tumor. (middle row) Image thresholding on a high CD8 cell infiltration into an ovarian tumor. At a threshold of 1 noise can still be seen, which is removed at threshold 5. At a threshold of 10, significant data loss is observed. (bottom row) Ten cells from low, medium or high leukocyte infiltrate image stacks were chosen (arrows) which were entirely within the volume scanned, and their antigen volume calculated. The mean cell volume was divided into the total fluorescence volume at the selected threshold to enumerate leukocytes.