Table 3 Computer generated volumes for 10 individual cells (rows) after thresholding of grey scales 1–10 (columns) from an image stack with a high cell infiltrate. The mean Cell Antigen Volume (CAV) for the 10 cells is shown at each threshold, as well as the standard deviation of the Cell Antigen Volume (SD CAV), the number of cells calculated for the image stack at each threshold, and the Total Antigen Volume (TAV) in the image stack at the corresponding threshold value.
From: Enumeration of leukocyte infiltration in solid tumors by confocal laser scanning microscopy
High Cell\Threshold | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|---|
1 | 1741 | 1248 | 977 | 696 | 539 | 461 | 340 | 262 | 198 | 162 |
2 | 1541 | 1000 | 733 | 577 | 458 | 386 | 325 | 286 | 236 | 191 |
3 | 1618 | 1071 | 801 | 597 | 413 | 334 | 253 | 176 | 116 | 79 |
4 | 1951 | 1261 | 900 | 675 | 504 | 399 | 317 | 243 | 197 | 146 |
5 | 1484 | 1098 | 818 | 638 | 475 | 369 | 287 | 223 | 163 | 123 |
6 | 2402 | 1593 | 1169 | 893 | 695 | 559 | 458 | 382 | 288 | 243 |
7 | 2621 | 1853 | 1371 | 1070 | 856 | 689 | 586 | 473 | 386 | 319 |
8 | 1016 | 739 | 584 | 466 | 405 | 310 | 236 | 169 | 111 | 77 |
9 | 1951 | 1393 | 1046 | 830 | 705 | 561 | 479 | 401 | 329 | 279 |
10 | 2439 | 1578 | 1116 | 772 | 537 | 388 | 289 | 209 | 140 | 109 |
Mean CAV (μm3) | 1876 | 1283 | 951 | 721 | 559 | 446 | 357 | 282 | 217 | 173 |
SD CAV (μm3) | 500 | 329 | 232 | 175 | 147 | 121 | 113 | 103 | 93 | 84 |
Number of Cells | 685 | 700 | 714 | 735 | 716 | 762 | 772 | 796 | 847 | 874 |
TAV (μm3 * 10E6) | 1.29 | 0.90 | 0.68 | 0.53 | 0.40 | 0.34 | 0.28 | 0.22 | 0.18 | 0.15 |