Figure 1
Influence of Pro-Th1 (IL-2, IL-12, IFN-γ) and Pro-Th2 (IL-4) cytokines on the proliferation and IFN-γ secretion when B cells, splenic macrophages and peritoneal macrophages were used as APCs. pGL-10 Th1 cells were cultured with B cells, splenic and peritoneal macrophages and ovalbumin (200 μg/ml). Cytokines IL-2, IL-4, IL-12 and IFN-γ were also added in the cultures. For proliferation, the cultures were pulsed after 48 h with 3H-thymidine (0.5 μCi/well) and harvested after last 16 h of incubation period. Data are expressed as mean ± SEM of triplicate wells (Fig. 1a). For IFN-γ, the supernatants were collected from the triplicate wells after 48 h of the initiation of cultures, pooled and estimated by ELISA. (Fig. 1b). The control cultures comprising of Th1 cells, Th1 cell+Ag, APCs+Ag, APCs+Th1 cells showed no discernible change. The data presented are from three independent experiments. '*' Represents p < 0.001 and '◆' represents p < 0.05. SMQ and PMQ represent splenic and peritoneal macrophages respectively.