Figure 3
Influence of the potency of first and second signals in the proliferation and IFN-γ secretion by Th1 cells. Cultures were set with pGL-10 Th1 cells (5 × 104/well) and the indicated concentrations of plate-bound anti-CD3 mAb (0.01 μg/ml) and in the presence of different concentrations of CHO-B7-1 (1 × 103/well, 1 × 104/well, 5 × 104/well). The cultures were pulsed after 48 h with 3H-thymidine (0.5 μCi/well) and harvested after last 16 h of incubation period. Data are expressed as mean ± SEM of triplicate wells (Fig. 3a). Production of IFN-γ (Fig. 3b) was estimated by ELISA in the pooled culture supernatants collected from the triplicate wells after 48 h of the initiation of cultures. The data is expressed as ng/ml. The control cultures comprising Th1 cells, Th1 cells+CHO-B7-1, Th1 cells+CHO cells, CHO-B7-1 cells+anti-CD3 Ab, CHO-B7-1 cells showed no noticeable change. The data presented are from three independent experiments. '*' Represents p < 0.001.