Figure 4
Influence of the potency of first and second signals on the proliferation of Th2 cells and secretion of IL-4 and IL-5. Cultures were set with (5 × 104/well) D10G4.1 Th2 cells and the indicated concentrations of plate-bound anti-CD3 mAb (0.01 μg/ml) and in the presence of different concentrations of CHO-B7-1 (1 × 103/well, 1 × 104/well, 5 × 104/well). For proliferation (Fig. 4a), all the cultures were pulsed after 48 h with 3H-thymidine (0.5 μCi/well) and harvested after the last 16 h of incubation period. Data are expressed as mean ± SEM of triplicate well. Production of IL-4 (Fig. 4b) and IL-5 (Fig. 4c) was estimated by ELISA. The supernatants were pooled from the triplicate wells after 48 h of the initiation of cultures. The data are expressed as pg/ml. The control cultures comprising of Th2 cells, Th2 cells+CHO-B7-1, Th2 cells+CHO cells, CHO-B7-1 cells+anti-CD3 Ab, CHO-B7-1 cells showed no remarkable change. The data presented are from four independent experiments. '*' Represents p < 0.001.