Figure 6
Influence of Pro-Th1 (IL-2 and IFN-γ) and Pro-Th2 (IL-4) cytokines on the proliferation and IL-4 and IL-5 secretion by Th2 cells stimulated with anti-CD3 Ab and B7-1. D10G4.1 Th2 cells were cultured with anti-CD3 Ab (0.01 μg/ml) in the presence of CHO-B7-1 (5 × 104/well). Cytokines IL-2, IL-4 or IFN-γ alone or in combinations were also added in to the cultures. For the proliferation (Fig. 6a), cultures were pulsed after 48 h with 3H-thymidine (0.5 μCi/well) and were harvested after last 16 h of incubation period. Data are expressed as mean ± SEM of triplicate wells. IL-4 (Fig. 6b) and IL-5 (Fig. 6c) were estimated by ELISA in the pooled supernatants collected from the triplicate wells after 48 h of the initiation of the cultures. The control cultures comprising Th2 cells, CHO-B7-1+Th2 cells, CHO+Th2 cells, CHO-B7-1, CHO showed no considerable change except that IL-5 secretion was 1995 pg/ml when D10G4.1 Th2 cells were incubated with CHO-B7.1 in the absence of anti-CD3 Ab. The data presented are from three independent experiments. '*' Represents p < 0.001, '†' represents p < 0.01 and '◆ ' represents p < 0.05.