Table 1 Primers for Real-Time PCR1
From: Profiling helper T cell subset gene expression in deer mice
Gene | Forward | Reverse | Size (bp) | Acc# |
|---|---|---|---|---|
GAPDH | GGTGCCAAAAGGGTCATCATCTC | GCAGGAAGCGTTGCTGATAATCTTG | 114 | AY841947 |
IFNγ | GGCTATTTCTGGCTGTTACTGCC | ATCCCCGACATCTGAGCTACTTG | 94 | AY289494 |
TNF | TGTAGCCCACGTTGTAGCAAACC | CTGGTTGTCTTTGAGATCCATGC | 106 | AF307013 |
LTA | ATGGTGTCTCCCATCTACACTTCAG | TTGAAACGGTCAGCATGGAGG | 115 | AF348259 |
IL-4 | CCCCGTGCTTGAAGAACAATTC | GGACTCATTCCCAGTACAGCTTTTC | 104 | DQ446203 |
IL-5 | GAAGAATCAAACTGTCCGTGGG | ACACTGCTCTTTTTGGCGGTC | 94 | AY843530 |
TGFβ1 | CGTGGAACTCTACCAGAAATACAGC | TCAAAAGACAACCACTCAGGCG | 96 | AY455973 |
IL-10 | TAAGGGTTACCTGGGTTGCCAAG | CAAATGCTCCTTGATTTCTGGGC | 106 | AF307012 |
GATA3 | AGTCCGCATCTCTTCACCTTCC | GGCACTCTTTCTCATCTTGCCG | 112 | AY325113 |
T-bet | GATCATCACTAAGCAAGGACGGC | AGACCACATCCACAAACATCCTG | 101 | AY271903 |
Fox-p3 | AAGCAGATCACCTCCTGGATGAG | TAGCACCCAGCTTCTCCTTTTCC | 114 | AY841945 |
STAT4 | AACCATTTACCTTCTGGACCTGG | TTGCTCACGAAGCCCATGATGTACC | 103 | AY455975 |
STAT6 | CGCTTTAGCGACTCTGAGATTGG | TCTTTGGCAGAAAATGGCTGG | 104 | DQ836357 |