Figure 5

The effect of cell subset depletion on the IFN-γ and IL-12 response. Splenocytes from naïve BALB/c mice were subjected to CD4⁺, CD8⁺, DX5⁺, Gr-1⁺ or Ly-6G⁺ cell depletion by MACS and treated with heat-killed or live B. pseudomallei strain KHW. PMA and ionomycin stimulation served as positive controls, and the untreated total splenocytes served as negative controls. IFN-γ (A) and IL-12 (B) concentrations were determined by ELISA from supernatants harvested at 24 h after stimulation. Results shown are representative of at least 3 experiments.