Table 1 Prevalence of cytoplasmic, nucleoplasmic and NE antibodies by immunofluorescence using various substrates and fixation schemes in 33 PBC patients' sera.
From: Optimized detection of circulating anti-nuclear envelope autoantibodies by immunofluorescence
Cell type/fixation | autoantigen localization | |||||
|---|---|---|---|---|---|---|
| Â | Cytoplasmic | nucleoplasmic | NE | |||
| Â | No. | % | No. | % | No. | % |
HeLa/4%FA | 19 | 57.5 | 10 | 30.3 | 11 | 33.3 |
HeLa/1%FA | 9 | 27.2 | 12 | 36.4 | 13 | 39.4 |
Hep2/4%FA | 25 | 75.7 | 13 | 39.4 | 11 | 33.3 |
Hep2/1%FA | 10 | 30.3 | 15 | 45.4 | 15 | 45.4 |
Hep2 Inova | 18 | 54.5 | 13 | 39.4 | 5 | 15.1 |
Hep2 bmd | 19 | 57.5 | 12 | 36.4 | 4 | 12.1 |
Hep2 Zeus | 17 | 51.5 | 18 | 54.5 | 7 | 21.2 |