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Figure 2 | BMC Immunology

Figure 2

From: Targeted in vitro and in vivo gene transfer into T Lymphocytes: potential of direct inhibition of allo-immune activation

Figure 2

Construction and efficacy of p21-EGFP construct. p21-EGFP constructs were prepared by ligating p21 gene construct in Multiple Cloning Site of EGFP vector. Colonies were picked and amplified for p21 and GFP gene expression. A. The results of PCR amplification of two representative colonies are shown. DNA from colony #2 (lanes 2 and 4) exhibits stronger gene expression for both p21 and GFP compared to colony #1 (lanes 1 and 3). More DNA was prepared from colony #2 and used to assess in vivo efficiency of transfection using intramuscular injection. B: Efficacy of intramuscular injection of p21-GFP plasmid DNA is shown as p21 and GFP mRNA expression in splenocytes individually from 5 mice.

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BMC Immunology

ISSN: 1471-2172

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