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Figure 3 | BMC Immunology

Figure 3

From: Targeted in vitro and in vivo gene transfer into T Lymphocytes: potential of direct inhibition of allo-immune activation

Figure 3

Efficacy of CD3-p21 chimeric plasmid DNA. The efficacy of CD3-p21 plasmid DNA in mouse Thyoma cells. T cells were transfected as described in Material and Methods section, and studied for presence of p21 mRNA and protein expression. A: p21 mRNA expression: The expression of p21 mRNA is seen only in cells transfected with CD3Sp21 sense plasmid DNA (lane 1) compared to cells transfected with either CD3ASp21 plasmid DNA (lane 2) or the empty vector DNA (lane 3). An equal expression of β-actin, a house-keeping gene for these three representative cell lines is also shown. B: p21 protein expression: Comparisom of expression of p21 protein in the control DNA (lane 1), CD3Sp21 (lane 2) and CD3ASp21 (lane 3) transfected cells, is shown in Western blot analysis of p21 protein. C: IL-2 mRNA expression: The expression of Il-2 mRNA in cells transfected with CD3Sp21 DNA is almost inhibited (lane 3) compared to cells transfected with the empty vector DNA (Lane 1), where as IL-2 mRNA is increased in CD3Asp21 DNA transfected cells (Lane 2). An equal expression of β-actin, a house-keeping gene for these three representative cell lines is also shown for each transfection. CD3Sp21 = CD3sensep21 and CD3ASp21 = CD3Antisensep21 plasmid DNA

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BMC Immunology

ISSN: 1471-2172

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