EGFP-p47phoxtranslocates to the plasma membrane in nucleoporated neutrophils. Neutrophils were nucleoporated with a vector expressing EGFP-p47phoxor EGFP control vector and recovered in RPMI. Two hours after transfection the specimens were transferred to a live chamber (37°C and 4.9% CO2) and cells were stimulated with PMA (0.1 μg/ml). The distribution of the fluorescent chimera was analyzed by confocal microscopy at the indicated intervals. The images were processed and analyzed for the distribution of the fluorescence intensity using the NIH image processing and analysis program IMAGE/J software. Scale bars = 3 μm.