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Figure 8 | BMC Immunology

Figure 8

From: Gene transfer and expression in human neutrophils. The phox homology domain of p47phox translocates to the plasma membrane but not to the membrane of mature phagosomes

Figure 8

Distribution of exogenously expressed p47phox-PX domain and RhoB during phagocytosis in HL-60 human promyelocytic cells HL-60 promyelocytic cells were differentiated to granulocytes and transfected with the indicated expression vector as described under "Methods." Phagocytosis of Texas Red-labeled opsonized-zymosan particles was conducted at 37°C for 15 min. B, The difference between the fluorescence intensity at the phagosome membrane versus that at the surrounding cytosolic area (ΔFI) was calculated using the NIH image processing and analysis program IMAGE/J software as described under "Methods." The results are mean ± SEM of three phagosomes from different cells.

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BMC Immunology

ISSN: 1471-2172

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