Figure 1

ELISA assays demonstrating the induction of a xenoantibody response following infusion with porcine hepatocytes. Two rhesus monkeys (#644 and #653) were infused with porcine hepatocytes, and the xenoantibody response was measured by ELISA. Panels (A.) and (B.) demonstrate the antibody response using pig aortic endothelial cells (PAEC) as antigenic targets. (A.) IgM and (B.) IgG. Panels (C.) through (F.) represent the anti-gal antibody response using purified di, tri, or penta αGal oligosaccharides as the antigenic target. (C.) #644 IgM. (D.) #653 IgM. (E.) #644 IgG. (F.) #653 IgG. Each sample was run in duplicate. Both IgM and IgG xenoantibodies against all tested antigenic targets were increased in each monkey post-porcine cell exposure.