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Figure 5 | BMC Immunology

Figure 5

From: β-(1→3)-D-glucan modulates DNA binding of nuclear factors κB, AT and IL-6 leading to an anti-inflammatory shift of the IL-1β/IL-1 receptor antagonist ratio

Figure 5

GP modulated TSST-1-induced DNA binding to a new NFATP2/3 site in the IL-1RA promoter. Binding was up-regulated for GP + LPS and GP + TSST-1, when compared to GP and, in the latter case, to both GP and TSST-1 (* = p < 0.05). Human PBMC were incubated with medium control, LPS, TSST-1, GP, GP + TSST-1 and GP + LPS for 1 h at 37°C. Schematic representation of the elevated NFAT-DNA binding activity to the site in the IL-1RA promoter. Bars depicting the theoretical values for GP/LPS calc. and GP/TSST-1 calc. are added. Medium control levels were set equal to 1 ± SEM (n = 6). An autoradiogram of a representative experiment is shown in the lower panel (lane 1: water; lane 2: medium control; lane 3: GP; lane 4: LPS; lane 5: TSST-1, lane 6: GP + LPS; lane 7: GP + TSST-1).

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BMC Immunology

ISSN: 1471-2172

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