β-(1→3)-D-glucan modulates DNA binding of nuclear factors κB, AT and IL-6 leading to an anti-inflammatory shift of the IL-1β/IL-1 receptor antagonist ratio

Table 1 Sense strand of oligonucleotides used in EMSAs.

Oligonucleotides^a	Base sequences (5'- XXX -3')	binding site/cytokine gene promoter (position of the cytokine gene from the TSS)^b
NFκBcS	GAT CCT CAG AGG GGA CTT TCC^c GAT G	NFκB consensus sequence/TNFα promoter [27]
NFκB1S	GAT CCT GGG ACA GCC C AG	NFκB1 sequence/TNFα promoter [27]
NFκB2S	GAT CCG GGG TAT CCT G	NFκB2 sequence/TNFα promoter [27]
NFκB3S	GAT CCT GGG TTT CTC C G	NFκB3 sequence/TNFα promoter [27]
IL-8κBcS	ATC GTG GAA TTT CCT CTG A	NFκB consensus sequence/IL-8 promoter [28]
NFATP2S	GAT CTA AAA TTT CCA GT C CTT GA	NFATP2 sequence/IFNγ promoter [29]
NFIL-6S	TGC AGA TTG CGC AAT CTG CA	NFIL-6 consensus sequence/IL-6 promoter [13]
NFκBcS	GCG AGG AGG GTA TTT CC G CTT	between -80 and -100 NFκB consensus/IL-1RA promoter [30]
NFκB3S	ACA ACA GCA AGG GTT TCT CT T TTT GGA AAT	between -100 and -130 NFκB3/IL-1RA promoter
NFκBcS	AGT AGG GAG TTT GG T	between -266 and -280 NFκB consensus sequence/IL-1RA promoter
NFκB2/3S	ACT CTG GGT ACC TGT	between -288 and -302 NFκB2/3 sequence/IL-1RA promoter
NFATP2/3S	GGC GCA CAA AAC CTA AAA TAT TT A CTA TCT	between -471 and -500 NFATP2/3 sequence/IL-1RA promoter
NFIL-6S	TTA CAA CAC TCC ATT GCG ACA C TT AGT GGG	between -140 and -170 NFIL-6 sequence/IL-1RA promoter [31]
Oct-1 DNAS	AAT TGC ATT GCC TGC AGG TCG ACT CTA GAG GAT CCA TGC AAA TGG ATC CCC GGG TAC CGA GCT C	exclusion of unspecific bindings, unrelated DNA, (Amersham Pharmacia)

ISSN: 1471-2172