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Figure 1 | BMC Immunology

Figure 1

From: Modulation of microglial/macrophage activation by macrophage inhibitory factor (TKP) or tuftsin (TKPR) attenuates the disease course of experimental autoimmune encephalomyelitis

Figure 1

Prophylactic MIF infusion does not alter onset, severity or recovery of EAE. (A) Wt mice were infused with MIF for 30 days, starting one day prior to EAE induction. The mice were weighed and scored daily for symptoms using the severity scale outlined in Methods. Daily scores were averaged. PBS n = 18; MIF d-1 n = 12. Wilcoxon test showed no statistically significant differences in onset, severity or recovery, but the progression of the disease is more delayed. (B) Frozen spinal cord sections were stained with Luxol Fast Blue to show areas of myelination. The dashed line and the asterisk demarcate the ventral column of the spinal cord. The intensity of luxol fast blue staining within the ventral column was quantified using the NIH Image freeware and was normalized to day 0 staining. Background staining was subtracted. (C) Immunohistochemistry for activated macrophage/microglial cells using F4/80 in frozen spinal cord sections. (D) Immunohistochemistry for infiltrating T cells using an anti-CD3 antibody.

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BMC Immunology

ISSN: 1471-2172

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