Therapeutic administration of tuftsin in tPA-/- mice does not alter onset, severity or recovery of EAE. (A) Wilcoxon test showed no significant difference in EAE parameters (p > 0.05). wt PBS n = 18, tPA-/- tufd7 n = 7. (B) Demyelination is visualized by luxol fast blue. The dashed line and the asterisk demarcate the ventral column of the spinal cord. The intensity of luxol fast blue staining within the ventral column was quantified using the NIH Image freeware and was normalized to day 0 staining. Background staining was subtracted. (C) F4/80+-reactive macrophages/microglia detected by immunohistochemistry. (D) Immunohistochemistry for T cells using an anti-CD3 antibody.