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Table 1 Effect of TSLP on thymocyte subpopulations in FTOC

From: TSLP is involved in expansion of early thymocyte progenitors

Cell # (×103)a

Medium

TSLP 10 ng/ml

TSLP 30 ng/ml

TSLP 100 ng/ml

Total

205.7 +/- 73.4b

220 +/- 32.5

356.7 +/- 21.9*

513.3 +/- 72.5**

 

(1×)c

(1×)

(1.7×)

(2.5×)

CD4-CD8+

31.0 +/- 9.5

47.3 +/- 8.7

72.3 +/- 15.9*

103.7 +/- 16.9**

 

(1×)

(1.5×)

(2.3×)

(3.3×)

CD4+CD8+

138.5 +/- 51.3

124.3 +/- 18.3

169.4 +/- 5.6

260.9 +/- 17.6*

 

(1×)

(1×)

(1.2×)

(1.9×)

CD4-CD8-

18.7 +/- 8.0

34.1 +/- 5.4*

75.7 +/- 1.2**

96.2 +/- 23.0**

 

(1×)

(1.8×)

(4.0×)

(5.1×)

CD4+CD8-

17.5 +/- 6.9

14.3 +/- 1.3

39.3 +/- 0.4**

52.3 +/- 18.6*

 

(1×)

(1×)

(2.2×)

(3.0×)

DN1d

0.6 +/- 0.2

1.6 +/- 0.3*

3.0 +/- 0.5**

5.7 +/- 0.9**

 

(1×)

(2.7×)

(5.0×)

(9.6×)

DN2

1.9 +/- 1.0

6.4 +/- 1.8*

9.6 +/- 5.3**

19.4 +/- 2.5**

 

(1×)

(3.4×)

(5.1×)

(10.3×)

DN3

10.6 +/- 5.0

12.0 +/- 3.4

31.2 +/- 8.3

26.7 +/- 16.5

 

(1×)

(1×)

(2.9×)

(2.5×)

DN4

0.9 +/- 0.4

2.0 +/- 0.4*

6.5 +/- 1.1**

5.5 +/- 1.5**

 

(1×)

(2.2×)

(6.9×)

(5.9×)

  1. a E15 FTOC with different doses of TSLP were performed for 2 weeks. Thymocytes were harvested and analyzed by FACS. The number (×103) of thymocyte subsets recovered from each FTOC lobe is shown. The data is a summary of 4 independent experiments.
  2. b Average number +/- SD;
  3. c Fold over medium control.
  4. d Thymocytes that were negative for expression of CD3/CD4/CD8 were analyzed for DN1 to DN4 subpopulations based on the expression of CD44 and CD25. * p < 0.05; ** p < 0.01, compared with medium control.