Figure 6
From: CD8α is expressed by human monocytes and enhances FcγR-dependent responses
Subtle differences in CD8α between monocytes and lymphocytes detected by 2-D electrophoresis. A, Western blot with a polyclonal anti-CD8α antibody after 2-D electrophoresis of ex vivo monocytes and lymphocytes. Cell lysates of lymphocytes and monocytes from one donor were separated by adherence, halved and treated (bottom panels) or not treated (top panels) with neuraminidase before analysis. B, Western blot with anti-CD8α mAb D9. C, Neuraminidase treatment and western blot with D9 of lymphocyte lysate. Results are representative of experiments with three donors.