Figure 1
From: Perforin enhances the granulysin-induced lysis of Listeria innocua in human dendritic cells
Effect of granulysin and perforin treatment on L. innocua in human DC, DC viability, and L. innocua in suspension. (A) L. innocua-challenged DC were incubated for 3 hours with granulysin or actinfrag as a control in varying concentrations in presence or absence of 2.5 kU/ml perforin at 37°C. After granulysin incubation, the cells were lysed in ice-cold water and Listeria viability was assessed in CFU assays. Mean values and SD of four independent experiments are presented. (B) L. innocua-challenged DC were incubated with granulysin at indicated concentrations in presence or absence of 2.5 kU/ml perforin for 3 hours at 37°C. After the incubation LDH content was measured in the supernatant and specific lysis was calculated. Mean values and SD of three independent experiments are presented. (C) L. innocua was incubated with granulysin in indicated concentrations +/- 2.5 kU/ml perforin for 3 hours at 37°C. After the granulysin incubation Listeria viability was calculated from bacterial growth curves obtained by turbidimetry.