Figure 6
From: Perforin enhances the granulysin-induced lysis of Listeria innocua in human dendritic cells
Effect of perforin on granulysin transfer to early endosomes in DC. L. innocua-challenged DC were incubated with 2.5 μM granulysin (A) or with 2.5 μM granulysin and 2.5 kU/ml perforin (B) for 45 minutes at 37°C. After fixation, the cells were stained with an anti-His Ab and with a polyclonal Ab recognizing the early endosomal antigen 1 (EEA-1). Nuclear and bacterial DNA was labeled with DAPI (blue), early endosomes are marked in green, and granulysin is depicted in red. Colocalization of the EEA-1 (green) and granulysin (red) was additionally calculated and visualized using Imaris software (lower row), bar = 8 μm.