Figure 7
From: Perforin enhances the granulysin-induced lysis of Listeria innocua in human dendritic cells
Effect of perforin on granulysin transfer to L. innocua -containing phagosomes. Representative images of L. innocua-challenged DC that were incubated with 2.5 μM granulysin (A) or with 2.5 μM granulysin and 2.5 kU/ml perforin (B) for 3 hours at 37°C. After fixation, the cells were stained with an anti-His Ab. Nuclear and bacterial DNA was labeled with DAPI (green), and granulysin is depicted in red, colocalization of the DAPI and granulysin was calculated and visualized using Imaris software (lower row), bar = 8 μm. At indicated time points colocalization of bacterial DNA with the granulysin staining was quantified in four independent experiments (C) Mean values and SD of colocalization in all evaluated cells per time point of four independent experiments are presented.