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Figure 11 | BMC Immunology

Figure 11

From: Transcription factor network downstream of protease activated receptors (PARs) modulating mouse bladder inflammation

Figure 11

Electrophoretic mobility shift (EMSA) and gel shift assays. A) EMSA was performed with C57BL/6 mice treated intravesically with control peptide, PAR1-AP, PAR2-AP, or PAR4-AP, at a concentration of 10 μM. Following treatment, the bladders were removed and the urothelium/submucosa was separated from the detrusor smooth muscle, and nuclear extracts were obtained from both layers. Shifted bands were observed with all treatments. C) No significant increase was observed in mast cell deficient Kitw/Kitw-v mice challenged with PAR1-AP. For super-shift assays (B and D), reactions were incubated for 30 minutes at 4°C with TFEB antibody, prior to addition of the probe.

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