Table 3 Primer and probes used for real-time RT-PCR
Gene | Forward-Primer | Reverse-Primer | TaqMan-Probe |
|---|---|---|---|
Act | CAGCGGAACCGCTACTTGCCAATGG | TCACCCACACTGTGCCCATCTACGA | ATGCCCTCCCCCATGCCATCCTGCGT |
IL-2 | GAATGGAATTAATAATTACAAGAATCCC | TGTTTCAGATCCCTTTAGTTCCAG | ATGCCCAAGAAGGCCACAGAACTG |
IL-3 | GCTCCCATGACCCAGACAAC | GGCAGACATGGCAGGAGATT | AGCTGGGTTAACTGCTCTAACATGATCGATGAAA |
IL-4 | CCCCCTCTGTTCTTCCTGCT | AGCCCTGCAGAAGGTTTCCT | TGCCGGCAACTTTGTCCACGG |
IL-5 | AGGATGCTTCTGCATTTGA | TTCTATTATCCACTCGGTGTTC | TATGCCATCCCCACAGAAATTCCCACA |
IL-13 | GGAGCTGGTCAACATCACCC | CGTTGATCAGGGATTCCAGG | CCAGAAGGCTCCGCTCTGCAATGGC |
IFNγ | CGAGATGACTTCGAAAAGCTGAC | CGCTTCCCTGTTTTAGCTGC | TCCAAGTGATGGCTGAACTGTCGCC |
GM-CSF | GCCCTGGGAGCATGTGAAT | GCTCCAGGCGGGTCTGTAG | AGGCCCGGCGTCTCCTGAACCT |