Figure 3

ICAM-1, ICAM-2, and ICAM-3 differentially promote memory cell development. Progression of naïve to memory cell differentiation was monitored by immunophenotyping of CD45RA, CD45RO in CD4+T cells over 14 days, with full CD45RO development observed by ICAM-2 stimulation in the presence of CD3/CD28. 5 × 10⁶ naïve CD4+ T cells were plated on 96-well plates coated with CD3/CD28 (control) or CD3/CD28 in the presence of α-LFA-1 antibody (clone TS1/22), ICAM-1, ICAM-2, ICAM-3, or combinations of ICAMs (as indicated). Cells were stained with CD45RA-PE, CD45RO-Cychrome, Annexin-FITC, and PI on days 1, 3, 5, 7, 9, 12, and 14. TruCount beads were used to obtain absolute cell counts. Data was computed and compiled using TreeView software for pattern recognition. The color intensities are averaged from triplicate experiments and each row represents an individual donor. Triplicate data for each of the three donors is displayed per stimulant. The experiment was repeated with naïve T cells from six donors.