Figure 6
From: Differential role of ICAM ligands in determination of human memory T cell differentiation
Generation of CD45RO+ memory T cell subsets with distinct CXCR3 expression and functional characteristics. (A) Chemokine surface receptor profiling of naïve CD4+ T cells stimulated with CD3/CD28, ICAM-1/CD3/CD28, ICAM-2/CD3/CD28, or ICAM-3/CD3/CD28 for 7 days. Cells were gated on either CD4+CD45RA+ (top row) or CD4+CD45RO+ (bottom row). Highlighted subset contour plots identify expression pattern and frequency for CXCR3 and CD11a on CD4+CD45RO+ cells. Flow cytometry plots are representative of triplicate measures from two individual donors. Repeating assay at lower concentrations of ICAM concentration showed no difference (data not shown). (B) Specific transmigration of naïve CD4+ T cells after 21 days of stimulation. Cells were differentiated in vitro with CD3/CD28, ICAM-1/CD3/CD28, ICAM-2/CD3/CD28, or ICAM-3/CD3/CD28 and subjected to a transmigration assay using specific chemoattractant molecules (as noted in Fig.). After 24 hours, cells were immunophenotyped for CD4+CD45RO+CD11aBrCD27+ (top panel) and CD4+CD45RO+CD11aBrCD27- (bottom panel). Experiments were reproduced with cells from two different donors. Transmigration experiments were repeated in duplicates with material from three donors. tTest calculations were performed and indicated in the table insert, with p < 0.05 being significant.