Table 1 Real time PCR primers and probes
Gene name | Accession No. | Universal Probe No. | Primer | Primer sequence |
|---|---|---|---|---|
B2M | NM_004048.2 | 42 | sense | ttctggcctggaggctatc |
non-sense | tcaggaaatttgactttccattc | |||
Beta Actin | NM_001101.2 | 11 | sense | attggcaatgagcggttc |
non-sense | ggatgccacaggactccat | |||
HPRT | NM_000194. | 73 | sense | tgaccttgatttattttgcatacc |
non-sense | cgagcaagacgttcagtcct | |||
L14 | BC000606 | 8 | sense | tcctcaagtttccgcacagt |
non-sense | ggctgcccattttgtattga | |||
L32 | NM_000994.3 | 17 | sense | gaagttcctggtccacaacg |
non-sense | gcgatctcggcacagtaag | |||
SDHA | NM_004168.1 | 69 | sense | agaagccctttgaggagca |
non-sense | cgattacgggtctatattccaga | |||
YWHAZ | NM_003406.2 | 9 | sense | cgttacttggctgaggttgc |
non-sense | tgcttgttgtgactgatcgac | |||
GAPDH 3' | M33197_3 | 45 | sense | acacccactcctccaccttt |
non-sense | tgacaaagtggtcgttgagg | |||
GAPDH mid | M33197_M | 45 | sense | gggaaactgtggcgtgat |
non-sense | gatgaccttgcccacagc | |||
GAPDH 5' | M33197_5 | 9 | sense | ggaagcttgtcatcaatggaa |
non-sense | ttgattttggagggatctcg | |||
ICAM1 | NM_000201 | 10 | sense | agcttctcctgctctgcaac |
non-sense | aatccctctcgtccagtcg | |||
IL-6 | NM_000600 | 40 | sense | gatgagtacaaaagtcctgatcca |
non-sense | ctgcagccactggttctgt |